0000050927 00000 n >�8s��p([VǠ���]`�R1�������]�^5ҕ���-R�?��=� �v�I�VŴ]�6V�x]83%m�M� 0000049374 00000 n 0000079192 00000 n Genetic selection and screening By: Majid Mojarrad •Selection: –Exploitation of the genetics of a recombinant organism to enable desirable, recombinant genomes to be selected over non-recombinants during growth •Screening: –Identification of a clone in a genomic or cDNA library (q.v.) U�9;�+���o�7�2e�e�\��Y5q� ��>�o�m�n̛h��g2[��ԉè?��:qܳ��A8z�� Yz9�#�(O��#,����e��]a �kK�뒡Q_��,]�=�UG�5�H�8���}p�`�.��y?7g� 0000002355 00000 n Critères de qualité et limites de ce type de production 1. Selection is where some sort of pressure (e.g. 0000100079 00000 n Sur un milieu contenant du saccharose, seules les bactéries dont le gène sacB est modifié se développeront. 0000029090 00000 n 0000054818 00000 n 0000028686 00000 n PDF | On Oct 12, 2011, Sriram Padmanabhan and others published Screening of Bacterial Recombinants: Strategies and Preventing False Positives | Find, read and cite all … 0000058668 00000 n 0000001520 00000 n 66 0 obj << /Linearized 1 /O 70 /H [ 1734 621 ] /L 425744 /E 104098 /N 8 /T 424306 >> endobj xref 66 52 0000000016 00000 n 0000049464 00000 n 0000056741 00000 n Avantages III. ����T��dX@�"�`ur�*"qQ [����ܐ'��C[$��?˜��C���*/�6�t�|H��"��G&����8&�ڬ�Hxʊ�[�>;[�h�q��ع�ۧw��ԡ aT��͡���ʡ~v=�"��Fm�߿��!�I���R=��`rl��v�o����+���1OD�q����@�z�:E_����ŻM�K�m*�Dɜ�y��o����Ҿf�A�z��A��x=���ZH^[�ff��#q?N�q�6N�����g�t�����g����`z&O��c�7c\y��ߠݎ�$�[���� Anticorps humanisé 1. I) BLUE-WHITE SCREENING The … selection and screening of recombinants pdf Screening lambdagt recombinant clones by hybridization to single plaques in situ.A system for direct screening of recombinant clones in Lactococcus lactis, based on. 6�t-��BY� �~aC��_-y�y�ە����l|/���X�(���^�c�\|抷�Z|ب*Ԡ��9��h�1x�ݺ�Z�E���x��� �|��Qhnf���]:��M�̺��}�hBL�78�(���m�i*�_�ijE)����7b��X=��Ft� �Mj�p�'*8�[�/:�:�����Y��bK�.E�Ip��D]˖�`�0�?�m_��o?�}��M�t6�S���_U�}+�� ŷ&[3�+�m�&�}� ���я͓M���3J݃gR���'��Cn���"��9�N��r�A��eQH�&��i�:~`l H6A5��9�ue����Nϵ5�N�L}EЉ'��$�x�ca^U���+���;�[�R��!��ߓ܂��`�@�[��ئ��{/2���$42kCu�&+׫C�P�yh�? 0000003079 00000 n the presence of an antibiotic) is applied during the growth of host cells containing recombinant DNA. 0000002564 00000 n 0000098126 00000 n by using a method that discriminates between different clones. If using a high-copy plasmid, 1–5ml (more typically, 1–2ml) is plenty. 3. 0000078437 00000 n 0000058024 00000 n 0000002333 00000 n Immuno Analyse Enzymatique VI. Aerating the culture is very important for maximum cell density. )� �k�]%���upt��)��HUG�Ǣ�{�A�V��g�M��r@P�'x9R�{���=T&/���Q' �� z�ɠ>G���9�–���Ts�����T�A��=7�����"b��B���2�= l[Ƀ�:m���]�S\�s5 SCREENING OF RECOMBINANTS A genetic screen or mutagenesis screen is an experimental technique used to identify and select for individuals who possess a phenotype of interest in a mutagenised population. 0000057344 00000 n 0000098033 00000 n 0000001405 00000 n Critères de qualité 2. 0000001672 00000 n 0000056417 00000 n 0000102758 00000 n �A�m����m:� 4�)�� _f���(Gv�p,�u�;F���g�L�\�f��O���5� �)׾g�b��D)� Xƃ^�‘4c�H4~]�.�ɂ��^ƽ&������ie���[P��Q�b � endstream endobj 117 0 obj 512 endobj 70 0 obj << /Contents [ 87 0 R 89 0 R 91 0 R 93 0 R 95 0 R 97 0 R 103 0 R 106 0 R ] /Type /Page /Parent 63 0 R /Rotate 0 /MediaBox [ 0 0 612 792 ] /CropBox [ 0 0 612 792 ] /Resources 71 0 R >> endobj 71 0 obj << /ColorSpace << /Cs6 80 0 R /Cs9 81 0 R >> /Font << /F2 83 0 R /F5 76 0 R /F6 73 0 R /F17 101 0 R /F18 98 0 R >> /XObject << /Im5 104 0 R /Im34 115 0 R >> /ProcSet [ /PDF /Text /ImageB /ImageC /ImageI ] /ExtGState << /GS1 111 0 R /GS2 109 0 R /GS4 110 0 R >> >> endobj 72 0 obj << /Type /FontDescriptor /FontFile3 75 0 R /FontBBox [ -348 -559 1425 1054 ] /FontName /Marydale /Flags 4 /StemV 55 /StemH 52 /CapHeight 693 /XHeight 446 /Ascent 704 /Descent -625 /ItalicAngle 0 >> endobj 73 0 obj << /Type /Font /Encoding 77 0 R /BaseFont /Marydale /FirstChar 0 /LastChar 255 /Subtype /Type1 /ToUnicode 78 0 R /FontDescriptor 72 0 R /Widths [ 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 390 249 1000 636 1000 528 1000 1000 336 384 1000 579 237 471 201 357 630 393 534 534 561 615 495 555 630 595 231 237 1000 573 1000 567 579 609 633 729 663 648 582 865 690 234 573 1000 570 858 612 696 567 807 675 621 636 660 622 840 545 526 690 1000 1000 1000 1000 1000 1000 369 381 510 519 486 390 474 474 234 261 588 213 630 384 366 348 339 408 342 396 342 441 708 270 393 462 1000 1000 1000 1000 1000 387 1000 1000 1000 894 684 1000 1000 1000 1000 1000 1000 1000 342 300 1000 240 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 747 1000 1000 1000 1000 1000 1000 420 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 ] >> endobj 74 0 obj << /Type /FontDescriptor /FontFile3 79 0 R /FontBBox [ -348 -559 1430 1057 ] /FontName /Marydale-Bold /Flags 262148 /StemV 55 /StemH 52 /CapHeight 700 /XHeight 452 /Ascent 754 /Descent -679 /ItalicAngle 0 >> endobj 75 0 obj << /Length 21932 /Filter /FlateDecode /Subtype /Type1C >> stream trailer << /Size 118 /Info 64 0 R /Encrypt 68 0 R /Root 67 0 R /Prev 424296 /ID[<7c975f45481da35976d1dceb066a4ef4><714c8ac850a22e76732861c20dd6c43d>] >> startxref 0 %%EOF 67 0 obj << /Type /Catalog /Pages 63 0 R /Metadata 65 0 R /PageMode /UseThumbs /OpenAction 69 0 R >> endobj 68 0 obj << /Filter /Standard /R 2 /O ( U�V�.�`�����Dz�-���#_m�_�}�g) /U ([����z�Y7�����\(X�) /P -12 /V 1 /Length 40 >> endobj 69 0 obj << /S /GoTo /D [ 70 0 R /FitH -32768 ] >> endobj 116 0 obj << /S 434 /Filter /FlateDecode /Length 117 0 R >> stream Définition 2. 0000052788 00000 n antibiotic is depleted, the selection pressure to keep the plasmid is removed, and the plasmid may be lost.

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